https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43499 Wed 21 Sep 2022 10:05:07 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17834 Tue 26 Jun 2018 11:21:16 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23689 Thu 28 Oct 2021 13:03:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48973 Thu 20 Apr 2023 12:01:58 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:13284 Sat 24 Mar 2018 08:15:15 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:11169 Sat 24 Mar 2018 08:10:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18875 Sat 24 Mar 2018 08:03:15 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18462 3% and > 61% respectively. Peripheral blood monocytes were isolated (n = 61) and sputum macrophages were isolated from a subgroup of patients with asthma (n = 14), using immunomagnetic cell separation. RNA and nuclear proteins were extracted and quantified. Enzyme activity was assessed using fluorescent assays and gene expression of EP300, KAT2B, CREBBP, and HDACs 1, 2 and 3 were measured by qPCR. Results: There was a significant inverse association between blood monocyte HAT and HDAC activity (r = −0.58, P < 0.001). NA was associated with increased blood monocyte HAT enzyme activity (P = 0.02), decreased HDAC activity (P = 0.03), and increased HAT: HDAC ratio (P < 0.01) compared with eosinophilic asthma. There were no differences in gene expression of EP300, KAT2B, CREBBP, or HDACs 1, 2 and 3 in blood monocytes from subjects with asthma or inflammatory phenotypes of asthma. There was no effect of inhaled corticosteroid use, poor asthma control, or asthma severity on HAT/HDAC activities. Sputum macrophages had increased expression of KAT2B in eosinophilic compared with paucigranulocytic asthma. Conclusions and Clinical Relevance: Neutrophilic airway inflammation is associated with increased HAT and reduced HDAC activity in blood monocytes, demonstrating further systemic manifestations relating to the altered inflammatory gene transcription profile of neutrophilic asthma.]]> Sat 24 Mar 2018 07:59:46 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28316 Sat 24 Mar 2018 07:27:05 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23403 Mon 19 Aug 2024 09:48:18 AEST ]]>